Innate immunity gene expression profiles in conjunctival membrane biopsies from Amazonian buffalo

ABSTRACT Domestic buffalo production plays an economically important role in the Brazilian Amazon, but they are susceptible to many diseases favored by tropical climate and annually flooded habitats, including ocular diseases. In this context, it is select genotypes that maximize innate immunity Amazonian herds. We aimed characterise, for first time, gene expression profiles of immune system conjunctival membrane buffalo. Ocular tissue samples were collected from 60 clinically healthy slaughtered animals northern state Amapá. The histologically processed classification into three groups according quantitative degree lymphoid associated with conjunctiva (discrete, G1; slight, G2; moderate, G3 presence tissue). RT-PCR was used quantify inflammatory cytokine (IL6, IL10, TNFA, IFNG), Toll-like receptor 4 (TLR4), Defensin beta 110 (DEFB110), relative endogenous GAPDH gene. G1 presented low IL6, DEFB110, while G2 exhibited high IFNG, TLR4. All showed all tested genes. These results suggest a greater resistance pathogenic microorganisms buffalos group, proportion may be related individuals.